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Eiwitextractie


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Eiwitextractie

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Leverancier:  G-Biosciences
Omschrijving:   FOCUSâ„¢ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
UOM:  1 * 1 KIT

Leverancier:  ENZO LIFE SCIENCES
Omschrijving:   Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
UOM:  1 * 1 KIT
New Product

Leverancier:  ENZO LIFE SCIENCES
Omschrijving:   Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
UOM:  1 * 1 KIT
New Product

Leverancier:  G-Biosciences
Omschrijving:   InGelâ„¢ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM:  1 * 1 KIT

Leverancier:  G-Biosciences
Omschrijving:   Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
UOM:  1 * 1 KIT
Leverancier:  G-Biosciences
Omschrijving:   Protein-S-S-Reductantâ„¢ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductantâ„¢ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductantâ„¢ in place of DTT or β-mercaptoethanol and boil the sample.
Leverancier:  Thermo Fisher Scientific
Omschrijving:   The Pierceâ„¢ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Itemnummer: (ENZOBMLKI1761250)

Leverancier:  ENZO LIFE SCIENCES
Omschrijving:   FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM:  1 * 1,25 mL
New Product
Itemnummer: (786-599)

Leverancier:  G-Biosciences
Omschrijving:   Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM:  1 * 5 mL

Leverancier:  Thermo Fisher Scientific
Omschrijving:   Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM:  1 * 50 µG
Leverancier:  Thermo Fisher Scientific
Omschrijving:   Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM:  1 * 1 EA

Leverancier:  Thermo Fisher Scientific
Omschrijving:   Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM:  1 * 2 µG

Leverancier:  G-Biosciences
Omschrijving:   FOCUSâ„¢ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM:  1 * 1 KIT

Leverancier:  G-Biosciences
Omschrijving:   Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLifeâ„¢ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM:  1 * 1 KIT
Leverancier:  G-Biosciences
Omschrijving:   Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUTâ„¢ has been specifically developed for substantial removal of albumin from such samples.
Leverancier:  Thermo Fisher Scientific
Omschrijving:   Pierceâ„¢ TMTâ„¢ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het call nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11.
De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het call nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
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