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Eiwitextractie
Leverancier:
ENZO LIFE SCIENCES
Omschrijving:
Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.
Itemnummer:
(DGA01)
Leverancier:
G-Biosciences
Omschrijving:
The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM:
1 * 500 ST
Itemnummer:
(ENZOBMLKI1050300)
Leverancier:
ENZO LIFE SCIENCES
Omschrijving:
FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM:
1 * 300 µl
New Product
Itemnummer:
(PIER90067)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierceâ„¢ TMTzeroâ„¢ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM:
1 * 1 KIT
Itemnummer:
(786-018)
Leverancier:
G-Biosciences
Omschrijving:
FOCUSâ„¢ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
UOM:
1 * 1 KIT
Leverancier:
G-Biosciences
Omschrijving:
Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.
Itemnummer:
(PIER88279)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Pierceâ„¢ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM:
1 * 1 KIT
Itemnummer:
(786-246)
Leverancier:
G-Biosciences
Omschrijving:
Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
UOM:
1 * 1 KIT
Leverancier:
G-Biosciences
Omschrijving:
FOCUSâ„¢ PhosphoRichâ„¢ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUSâ„¢ PhosphoRichâ„¢ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.
Itemnummer:
(ENZOENZ450160100)
Leverancier:
ENZO LIFE SCIENCES
Omschrijving:
Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.
UOM:
1 * 1 KIT
New Product
Itemnummer:
(786-129)
Leverancier:
G-Biosciences
Omschrijving:
A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.
UOM:
1 * 1 KIT
Leverancier:
G-Biosciences
Omschrijving:
OrgoSol DetergentOUTâ„¢ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.
Itemnummer:
(PIER90051)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM:
1 * 20 µG
Leverancier:
Thermo Fisher Scientific
Omschrijving:
B-PERâ„¢ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
Leverancier:
G-Biosciences
Omschrijving:
Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.
Leverancier:
G-Biosciences
Omschrijving:
The F(ab)â‚‚ Fragmentation kits are designed for the generation and isolation of F(ab)â‚‚ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)â‚‚ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)â‚‚. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)â‚‚ fragments.
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 nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11.
De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het
nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
Bijkomende documentatie kan noodzakelijk zijn voor dit item. Een VWR-verantwoordelijke zal contact opnemen met u indien nodig.
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