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G Bioscienes
Fournisseur:
G-Biosciences
Description:
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
Numéro de catalogue:
(786-180)
Fournisseur:
G-Biosciences
Description:
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
UOM:
1 * 500 mL
Numéro de catalogue:
(R010)
Fournisseur:
G-Biosciences
Description:
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
UOM:
1 * 100 mL
Numéro de catalogue:
(R005)
Fournisseur:
G-Biosciences
Description:
Potassium chlorure 1 mol/l (1 N) en solution aqueuse
UOM:
1 * 100 mL
Numéro de catalogue:
(786-132)
Fournisseur:
G-Biosciences
Description:
The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.
Numéro de catalogue:
(786-325)
Fournisseur:
G-Biosciences
Description:
ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.
UOM:
1 * 50 Essai
Numéro de catalogue:
(786-14)
Fournisseur:
G-Biosciences
Description:
SG-Lysine-C™ endopeptidase, from <i>Lysobacter enzymogenes</i>, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
UOM:
1 * 2 ST
Fournisseur:
G-Biosciences
Description:
G-Biosciences' RAPIDstain™ is an ultra sensitive, ready-to-use protein stain based on Coomassie dye
Numéro de catalogue:
(786-229)
Fournisseur:
G-Biosciences
Description:
Ultra pure proteomic grade water that is fully compatible with 2D electrophoresis and mass spectrometry analysis.
UOM:
1 * 1 L
Numéro de catalogue:
(786-20LATEX)
Fournisseur:
G-Biosciences
Description:
A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
IgG elution buffer
Fournisseur:
G-Biosciences
Description:
X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.
Fournisseur:
G-Biosciences
Description:
The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.
Fournisseur:
G-Biosciences
Description:
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
Numéro de catalogue:
(RC-089)
Fournisseur:
G-Biosciences
Description:
Sodium acétate
UOM:
1 * 500 g
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