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Antibiotics & Antimycotics

Antibiotics and Antimycotics

Antibiotics and Antimycotics are a type of antimicrobial drug used in the treatment and prevention of bacterial infections. They may either kill or inhibit the growth of microorganisms.

In science those substances are mostly used in cell culture. They are used to select for a specific type of cells. Either when cultivating mammal cells to exclude bacteria or e.g. to select for genetically modified cells which carry a resistance gene for selection on them.

PanReac AppliChem antibiotics and antimycotics prove to be used from small assays to bigger incubators. On this page you can find a selection of our finest most common used products from this product range.

Also here it is about trust and reproducibility for all your experiments and growing in cell culture or microbiology.

List of selected antibiotics:

Product No. Description Target
organism		 Mode
of action		 Recommended
working
concentration		 Stock
solution

A1907

Amphotericin B

fungi, yeast

binds to sterols with planar structure and impairs with membrane permeability

0.25 µg/ml
>3 µg/ml fungicidal

30-40 mg/ml in DMSO

A3784

Blasticidin S hydrochloride

Prokaryotes, Eukaryotes

inhibits protein biosynthesis by preventing the formation of the peptide bond

3-100 µg/ml

50 mg/ml in H2O or buffer store at +4°C - -20°C

A1491

Carbenicillin disodium salt

gram negative germs, enterococci

inhibits cell wall synthesis (transpeptidase) in growing bacteria

20-60 µg/ml

50 mg/ml in H2O/MeOH 1:1 store at -20°C

A0879

Cycloheximide (Actidion)

fungi, eukaryotes

binds to 80 S ribosome in eukaryotic cells; inhibits formation of peptide bond

10 µg/ml

10 mg/ml

A1492

Gentamycin sulfate

gram positive/ negative germs

inhibits protein synthesis by binding to the L6 protein of the 50 S ribosomal subunit

15-50 µg/ml

10-20 mg/ml in water, formamide

A2175

Hygromycin B - Solution

    

10-400 µg/ml

ca. 41 mg/ml in H2O; store at -20°C

A1493 & A4789

Kanamycin sulfate & Kanamycin Sulfate (Ph. Eur., BP) pure, pharma rade

gram positive/
negative cocci & bacteria

inhibits protein synthesis (translocation)

10-100 µg/ml

10 mg/ml in H2O
store at -20°C

A0890

Polymyxin B sulfate

gram negative bacteria - non- proliferating germs too

interaction with phospholipid components of the bact. cell membrane; changes permeability of the membrane and causes efflux of essential plasma compounds

50 µg/ml

25 mg/ml H2O, MeOH

A1839

Vancomycin hydrochloride

bacteriostatic and bacterizide against gram positive cocci and bacteria

amphoteric glyopeptide antibiotic; binds to bacterial cell wall precursors (peptidoglycans)

1-25 µg/ml

soluble in water ›100 mg/ml

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