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Thermo Scientific restriction enzymes

The Great Double Digest Day

Double Digest your DNA in only 5-15 minutes with one buffer and load directly onto your gel with 176 Thermo Scientific FastDigest Enzymes.

Restriction digestion gets easy

Thermo Scientific™ FastDigest™ enzymes are a line of restriction enzymes that are all 100% active in a single buffer. The universal FastDigest and FastDigest Green Buffers allow single, double, or multiple DNA digestion within 5–15 minutes, eliminating any need for buffer changes or subsequent DNA clean-up steps. Thermo Scientific™ DNA-modifying enzymes have 100% activity in this buffer as well. The FastDigest Green Buffer includes a density reagent and two tracking dyes that allow for direct loading of digestion reaction products on gels.

  • 100% activity of all FastDigest enzymes in one buffer
  • Complete DNA digestion in 5–15 minutes
  • 100% buffer compatibility with downstream applications
  • Direct loading of reaction products on gels

Check the list of available Restriction Enzymes in the Thermo Scientific -Fast Digest restriction enzymes brochure below

Discover the trial size- FastDigest Value Pack

The Thermo Scientific™ FastDigest™ Value Pack (Cat. No. K1991) is a collection of 13 popular FastDigest enzymes supplied with FastDigest and FastDigest Green Buffers. Each enzyme is supplied in an amount sufficient for 20 standard restriction digestion reactions. The FastDigest enzymes included in the pack are: 
BamHI, BglII, EcoRI, EcoRV (Eco321), HindIII, KpnI, NdeI, NotI, PstI, SalI, SmaI, XbaI, and XhoI.

    Ordering information

    FastDigest Value Pack