23013
PIER23013EA
270
EUR
InStock
PIER23013
SPB (Succinimidyl-[4-(psoralen-8-yloxy)]butyrate), Pierce™
SPB (Succinimidyl-[4-(psoralen-8-yloxy)]butyrate)
Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.
- Reactive groups: NHS ester and psoralen
- Reactive toward: amino groups and thymine
- DNA intercalating agent and nucleic acid probe
- Reagent for photo-immobilization to microplates
Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ∼10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Opgelet: For Research Use Only. Not for use in diagnostic procedures.