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Protéines extraction
Numéro de catalogue:
(DGA01)
Fournisseur:
G-Biosciences
Description:
The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
UOM:
1 * 500 ST
Fournisseur:
Thermo Fisher Scientific
Description:
B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).
Fournisseur:
G-Biosciences
Description:
Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
Numéro de catalogue:
(786-598)
Fournisseur:
G-Biosciences
Description:
Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
UOM:
1 * 5 mL
Numéro de catalogue:
(786-022)
Fournisseur:
G-Biosciences
Description:
FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER90054)
Fournisseur:
Thermo Fisher Scientific
Description:
Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM:
1 * 50 µG
Numéro de catalogue:
(PIER90056)
Fournisseur:
Thermo Fisher Scientific
Description:
Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM:
1 * 1 EA
Numéro de catalogue:
(PIER90053)
Fournisseur:
Thermo Fisher Scientific
Description:
Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM:
1 * 2 µG
Numéro de catalogue:
(786-258)
Fournisseur:
G-Biosciences
Description:
Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
Numéro de catalogue:
(786-599)
Fournisseur:
G-Biosciences
Description:
Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM:
1 * 5 mL
Numéro de catalogue:
(ENZOBMLKI1761250)
Fournisseur:
ENZO LIFE SCIENCES
Description:
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM:
1 * 1,25 mL
New Product
Fournisseur:
Thermo Fisher Scientific
Description:
The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Numéro de catalogue:
(786-247)
Fournisseur:
G-Biosciences
Description:
FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM:
1 * 1 KIT
Numéro de catalogue:
(786-253)
Fournisseur:
G-Biosciences
Description:
FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.
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est toujours affiché et vous avez besoin d'aide, s'il vous plaît appelez-nous au 016 385 011
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