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Protéines extraction
Numéro de catalogue:
(786-790)
Fournisseur:
G-Biosciences
Description:
A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM:
1 * 1 ST
Numéro de catalogue:
(PIER88216)
Fournisseur:
Thermo Fisher Scientific
Description:
This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM:
1 * 1 ST
Fournisseur:
MP Biomedicals
Description:
Lysing Matrix Z is used primarily for lysis of animal and plant tissue, tougher stems, roots and seeds, and whole insects. Each impact-resistant tube contains yttria-stabilised zirconium oxide beads.
Numéro de catalogue:
(PIER89842)
Fournisseur:
Thermo Fisher Scientific
Description:
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER84868)
Fournisseur:
Thermo Fisher Scientific
Description:
Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.
UOM:
1 * 1 KIT
Fournisseur:
Thermo Fisher Scientific
Description:
The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
Fournisseur:
Thermo Fisher Scientific
Description:
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
Numéro de catalogue:
(786-248)
Fournisseur:
G-Biosciences
Description:
FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER89875)
Fournisseur:
Thermo Fisher Scientific
Description:
The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER85160)
Fournisseur:
Thermo Fisher Scientific
Description:
Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER89895)
Fournisseur:
Thermo Fisher Scientific
Description:
The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER89871)
Fournisseur:
Thermo Fisher Scientific
Description:
The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
UOM:
1 * 1 KIT
Numéro de catalogue:
(PIER89801)
Fournisseur:
Thermo Fisher Scientific
Description:
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
UOM:
1 * 1 KIT
Numéro de catalogue:
(786-792)
Fournisseur:
G-Biosciences
Description:
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM:
1 * 1 ST
Fournisseur:
Thermo Fisher Scientific
Description:
Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.
Fournisseur:
Thermo Fisher Scientific
Description:
The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).
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