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Réactifs pour acides nucléiques
Fournisseur:
Thermo Fisher Scientific
Description:
RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Numéro de catalogue:
(ICNA112200200)
Fournisseur:
MP Biomedicals
Description:
Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into <i>Pichia pastoris</i> for protein expression studies.
UOM:
1 * 250 Tests
Fournisseur:
FINNZYMES REAGENTS
Description:
Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Fournisseur:
FINNZYMES REAGENTS
Description:
Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.
Fournisseur:
Thermo Fisher Scientific
Description:
The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Fournisseur:
FINNZYMES REAGENTS
Description:
Phusion® High-Fidelity PCR kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.
Fournisseur:
FINNZYMES REAGENTS
Description:
DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.
Fournisseur:
FINNZYMES REAGENTS
Description:
Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
Fournisseur:
Thermo Fisher Scientific
Description:
Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.
Fournisseur:
FINNZYMES REAGENTS
Description:
Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than <i>Taq</i>.
Numéro de catalogue:
(ICNA116045050)
Fournisseur:
MP Biomedicals
Description:
FastRNA® Pro Green Kit is used to isolate total RNA from any type of plant and animal tissue or cultured cells. Extracted RNA is suitable for RT-PCR analysis and other downstream applications.
UOM:
1 * 1 KIT
Fournisseur:
Thermo Fisher Scientific
Description:
DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.
Numéro de catalogue:
(ICNA117022150)
Fournisseur:
MP Biomedicals
Description:
MPure™ Plant DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of genomic DNA from plant (leaf, seeds and spores) and fungal tissues. Up to 100 mg of tissue can be used for purification.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Also available separately.
Fournisseur:
G-Biosciences
Description:
GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml<i> E. coli </i>cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.
Numéro de catalogue:
(GZ-02)
Fournisseur:
G-Biosciences
Description:
The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of <i>E. coli</i>, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.
UOM:
1 * 1 KIT
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