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Western Blotting Reagents
Fournisseur:
G-Biosciences
Description:
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
Numéro de catalogue:
(J63209.AK)
Fournisseur:
Thermo Scientific
Description:
Saponin permeating solution, is used as a cell permeabilising agent.
UOM:
1 * 250 mL
Fournisseur:
Thermo Fisher Scientific
Description:
The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.
Numéro de catalogue:
(PIER15159)
Fournisseur:
Thermo Fisher Scientific
Description:
QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
UOM:
1 * 1 ST
Numéro de catalogue:
(TB110)
Fournisseur:
G-Biosciences
Description:
Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM:
1 * 1 ST
Fournisseur:
Thermo Fisher Scientific
Description:
The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.
Numéro de catalogue:
(A3417.5000)
Fournisseur:
PanReac AppliChem
Description:
Le CheLuminate-HRP PicoDetect est un kit complet comprenant des réactifs prêts à l'emploi pour la détection par chimiluminescence de protéines immobilisées (Western blot) ou d'acides nucléiques immobilisés (Southern ou Northern blot), conjugués à de la peroxydase de raifort (HRP) directement ou indirectement.
UOM:
1 * 1 KIT
Fournisseur:
Rockland Immunochemicals
Description:
These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.
Numéro de catalogue:
(RPN3006)
Fournisseur:
Cytiva
Description:
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM:
1 * 1 ST
Fournisseur:
Cytiva
Description:
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Fournisseur:
Cytiva
Description:
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Fournisseur:
Cytiva
Description:
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Fournisseur:
Cytiva
Description:
Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis. Cy5 Amidite can be used to prepare sequencing primers for use on ALF express.
Fournisseur:
Cytiva
Description:
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
Numéro de catalogue:
(RPN3005)
Fournisseur:
Cytiva
Description:
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM:
1 * 1 ST
Numéro de catalogue:
(RPN5785)
Fournisseur:
Cytiva
Description:
ECF™ substrate for Western blotting
UOM:
1 * 1 ST
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