90073
92000
90103
90104
90105
90106
90107
90108
90109
90110
90115
90116
92001
90073.EA
355
EUR
InStock
90073.
92000.
90103.
90104.
90105.
90106.
90107.
90108.
90109.
90110.
90115.
90116.
92001.
Reactive dye conjugates
Koppelings- en labelingssystemen voor eiwitten
Reactive dyes containing an amine functional group.
ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups. The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes. ARP can freely cross the cell membranes, thus allowing detection of abasic sites in living cells. ARP is suitable for use in microplate assays.
Aminooxy reagents, such as aminooxy-5(6)-FAM, react with aldehydes or ketones to form a stable hydrazone compound under mild conditions. Similar to ARP, aminooxy-5(6)-FAM can be used to label abasic sites in damaged DNA.
Dye conjugates to PEO3-amine have a long water-soluble PEG spacer that separates the fluorescent dye and the amine functional group. These dyes can be coupled to activated carboxy groups or sulfonyl chlorides. Cadaverine conjugates can be coupled to activated carboxy groups or sulfonyl chlorides.