02101905.2
0210190501
0210190505
ICNA02101905.2EA
27.4
EUR
InStock
ICNA02101905.2
ICNA0210190501
ICNA0210190505
Guanidiniumchloride, white powder, Practical grade
Guanidiniumchloride
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins.
This strong denaturant can solubilise insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins.