04820512
04820539
04820540
ICNA04820512EA
74.3
EUR
InStock
ICNA04820512
ICNA04820539
ICNA04820540
Guanidiniumchloride ≥99%, white crystalline powder, Ultrapure
Guanidiniumchloride
Guanidine Hydrochloride is a protein denaturant and thus having an important role in molecular weight determinations.
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.
- Ultra Pure Grade
- Suitable for molecular biology applications
- White Crystalline Powder
- pKa: 13.6 (Lit.)
- Store at Room Temperature (15-30 °C). Store Desiccated.
pH: 4 - 6 (6.0 M aq soln) (Lit.)