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Milieux de culture cellulaire


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Milieux de culture cellulaire

Cell culture media maintains the right pH and supports the growth of many types of cells. The media formulation includes amino acids, vitamins, glucose, inorganic salts and other growth factors designed to support the growth of cells or microorganisms


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Fournisseur:  VWR Chemicals
Description:   VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.
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Fournisseur:  VWR Chemicals
Description:   Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.
Fournisseur:  VWR Chemicals
Description:   Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.
Fournisseur:  VWR Chemicals
Description:   Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

Fournisseur:  VWR Chemicals
Description:   Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from <i>Drosophila spp</i> and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.
UOM:  1 * 500 mL
Numéro de catalogue: (392-0406)

Fournisseur:  VWR Chemicals
Description:   VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.
UOM:  1 * 100 mL
Numéro de catalogue: (392-0421)

Fournisseur:  VWR Chemicals
Description:   Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.
UOM:  1 * 500 mL
Numéro de catalogue: (392-0441)

Fournisseur:  VWR Chemicals
Description:   L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture.
UOM:  1 * 100 mL
Numéro de catalogue: (392-0418)

Fournisseur:  VWR Chemicals
Description:   Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.
UOM:  1 * 500 mL
Numéro de catalogue: (392-0420)

Fournisseur:  VWR Chemicals
Description:   McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
UOM:  1 * 500 mL
Fournisseur:  Biowest
Description:   McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.
Fournisseur:  HyClone products (Cytiva)
Description:   HyClone™ SFM4MAb media was developed through the Metabolic Pathway Design approach to increase process yields for manufacturing of human and humanised recombinant antibodies for therapeutic use.
Fournisseur:  HyClone products (Cytiva)
Description:   HyClone™ SFMTransfx-293 is a serum-free, animal derived component-free medium designed to support the growth of HEK 293 cultures and promote transfection using lipofection or similar methods. Manufactured according to cGMP guidelines.

Fournisseur:  HyClone products (Cytiva)
Description:   Ham's F-10 was designed for use with Chinese Hamster Ovary (CHO) cells, and has been shown to support the growth of human diploid cells, white blood cells, and primary explants from rat, rabbit, or chicken tissues. This classical media can be used for serum-free growth of some cell lines including CHO cells. Serum supplementation may or may not be necessary depending on the type of cell being cultured. Ham's F-10 contains a wide variety of components, including zinc, hypoxanthine, and thymidine and is buffered with sodium bicarbonate.
UOM:  1 * 500 mL
Fournisseur:  HyClone products (Cytiva)
Description:   HyClone Classical Liquid Media: Iscove's Modified Dulbeccos Medium (IMDM) is manufactured using ISO 9001:2000-certified processes.
Fournisseur:  BIOCONCEPT
Description:   Hybridoma growth media (HYGM) 7 is a serum-free, protein free, chemically defined (CD) and fully defined media that can be used for the cultivation of various different hybridomas and the production of monoclonal antibodies.
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