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Réactifs d'électrophorèse
Fournisseur:
G-Biosciences
Description:
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
Fournisseur:
Thermo Fisher Scientific
Description:
The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.
Numéro de catalogue:
(J67051.XI)
Fournisseur:
Thermo Scientific
Description:
This wide range molecular weight marker contains 60 ng/μl of 21 fragments from 100 bp to 12000 bp. The bands appear in 100 bp increments from 100 to 1000 bp and in 1000 bp increments from 1 to 12 kb. Bands at 1 kb and 5 kb stain brighter to aid identification.
UOM:
1 * 0,5 mL
Numéro de catalogue:
(J62931.8U)
Fournisseur:
Thermo Scientific
Description:
Ethidium bromide destaining bags are used to safely remove ethidium bromide from nucleotide solutions and gels during overnight treatment. Each bag extracts up to 5 mg of Ethidium Bromide from solution, thus minimising the exposure of research personnel to this material.
UOM:
1 * 25 ST
Numéro de catalogue:
(J63209.AK)
Fournisseur:
Thermo Scientific
Description:
Saponin permeating solution, is used as a cell permeabilising agent.
UOM:
1 * 250 mL
Fournisseur:
Rockland Immunochemicals
Description:
TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.
Numéro de catalogue:
(A5191.0025)
Fournisseur:
PanReac AppliChem
Description:
This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.
UOM:
1 * 0,25 mg
Fournisseur:
Merck Millipore (Novagen)
Description:
Les échelles Perfect DNA™ contiennent des jeux d'espèces d'DNA de taille pratique et aisément mémorisable pour l'analyse de gel d'agarose. Les marqueurs ont des intensités de bande uniformes et couvrent une large gamme de tailles d'DNA.
Numéro de catalogue:
(LONZ50334)
Fournisseur:
LONZA
Description:
QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.
UOM:
1 * 50 ST
Fournisseur:
Biotium
Description:
A visible three-colour protein marker for SDS-PAGE or western blotting, with 10 bands ranging from 10 to 180 kDa.
Numéro de catalogue:
(PIER15159)
Fournisseur:
Thermo Fisher Scientific
Description:
QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
UOM:
1 * 1 ST
Numéro de catalogue:
(69959-3)
Fournisseur:
Merck Millipore (Novagen)
Description:
The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.
UOM:
1 * 1 ST
Fournisseur:
LONZA
Description:
ProSieve™ Color protein marker have been labelled with three different fluorescent dyes. These markers are supplied as a lyophilised solid containing the buffer salts and detergent found in the typical Laemmli buffer system. It contains nine proteins with approximate masses of 10, 15, 20, 25, 40, 50, 80, 125, and 190 kDa.
Numéro de catalogue:
(TB110)
Fournisseur:
G-Biosciences
Description:
Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM:
1 * 1 ST
Fournisseur:
Thermo Fisher Scientific
Description:
The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.
Fournisseur:
Rockland Immunochemicals
Description:
These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.
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