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cell+culture+flasks
Numéro de catalogue:
(PRSI33-473)
Fournisseur:
ProSci Inc.
Description:
This antibody neutralises human TNFa mediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of hTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
UOM:
1 * 1 EA
New Product
Fournisseur:
Sartorius
Description:
The Vivapure® AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for<i> in vitro</i> transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.
Fournisseur:
STEMCELL Technologies
Description:
Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and the development of the immune system (Lyman <i>et al.</i>; Rosnet <i>et al.</i>). Flt3/Flk-2 ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, GM-CSF, and TNF-α, Flt3/Flk-2 ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler and Quentmeier; Wodnar-Filipowicz). Flt3/Flk-2 ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet <i>et al.</i>). Flt3/Flk-2 ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed outside the hematopoietic system in the brain, placenta, and testis (Drexler and Quentmeier; Hannum <i>et al.</i>).
Fournisseur:
Spectrum Chemical
Description:
HEPES, cGMP Manufactured is a zwitterionic biological buffer and is one of Good's buffers widely used in cell culture. Non-graded HEPES, cGMP Manufactured supplied by Spectrum is indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use.
Fournisseur:
STEMCELL Technologies
Description:
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf and Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit <i>et al.</i>), the activation of metalloproteases that cleave VCAM-1 (Levesque <i>et al.</i>), and the release of norepinephrine from the sympathetic nervous system (Katayama <i>et al.</i>), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola <i>et al.</i>). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya <i>et al.</i>). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.
Numéro de catalogue:
(734-1099)
Fournisseur:
Corning
Description:
La laminine, l'un des principaux composants des membranes basales, est à l'origine de différentes activités biologiques, elle favorise notamment l'adhésion, la migration, la croissance et la différenciation des cellules, y compris la formation de neurites. Elle peut être utilisée comme fin revêtement des surfaces des cultures tissulaires ou comme additif soluble à un milieu de culture. Le complexe laminine/entactine haute concentration est une formulation spéciale développée pour la culture tridimensionnelle (3D).
UOM:
1 * 1 mg
Fournisseur:
REVCO TECHNOLOGIES
Description:
With precise temperature control and stability of all culture parameters, the Series 8000 direct-heat CO₂ incubators offer dependable flexibility for growing cells. The automatic high-temperature decontamination cycle is ideal for overnight sterilisation and ensures consistent sterilisation time after time. Units are light weight and easy to move with accessory roller base, there is a choice of TC or IR CO₂ sensor.
Fournisseur:
MP Biomedicals
Description:
Storage: -20 °C.
Adenosine 5'-triphosphate (ATP) and its phosphate bonds are the basic components of energy exchange in many biological systems. Adenosine 5'-triphosphate (ATP) is a central component of energy storage and metabolism in vivo. ATP is used in many cellular processes, respiration, biosynthetic reactions, motility, and cell division. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase(s) that produce the second messenger cAMP. ATP provides the metabolic energy to drive metabolic pumps. ATP serves as a coenzyme in a wide array of enzymatic reactions. P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems.
Numéro de catalogue:
(734-1273)
Fournisseur:
Corning
Description:
La laminine, l'un des principaux composants des membranes basales, est à l'origine de différentes activités biologiques, elle favorise notamment l'adhésion, la migration, la croissance et la différenciation des cellules, y compris la formation de neurites. Elle peut être utilisée comme fin revêtement des surfaces des cultures tissulaires ou comme additif soluble à un milieu de culture. Le complexe laminine/entactine haute concentration est une formulation spéciale développée pour la culture tridimensionnelle (3D).
UOM:
1 * 10,5 mg
Numéro de catalogue:
(PRSI50-254)
Fournisseur:
ProSci Inc.
Description:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
UOM:
1 * 100 µl
Numéro de catalogue:
(PRSI33-216)
Fournisseur:
ProSci Inc.
Description:
This antibody is specific for a 17-26 kDa protein, which is identified as the cytokine TNF alpha (Tumor Necrosis Factor alpha). It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. It is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. It exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
UOM:
1 * 1 EA
New Product
Fournisseur:
MP Biomedicals
Description:
Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells. Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles. Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.
Numéro de catalogue:
(PRSI6221)
Fournisseur:
ProSci Inc.
Description:
LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-kappa B during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.
UOM:
1 * 1 EA
Fournisseur:
ELGA LabWater
Description:
L'Ultra se décline en 5 versions qui répondent spécifiquement aux différents besoins des laboratoires. Ultra Scientifique délivre de l'eau ultrapure en continu. Son pack de 2 cartouches assure en permanence une eau qui répond aux applications les plus exigeantes des scientifiques. Ultra Ionique répond aux exigences d'applications aussi critiques que celles de la chromatographie ou de la spectrophotométrie par l'élimination des fractions ioniques et organiques résiduelles de l'eau prétraitée. Ultra Analytic par l'intégration d'une lampe UV 185 nm, produit une eau dont le niveau de COT est exceptionnellement bas (<2 ppb). Affichage du niveau de COT en ppb. Ultra Bioscience assure la production d'une eau ultrapure apyrogène (fécondation in vitro, cultures cellulaires). Ultra Genetic intègre une lampe UV 185 nm et un module d'ultrafiltration. Affichage du niveau de COT en ppb.
Numéro de catalogue:
(734-0099)
Fournisseur:
Corning
Description:
Le collagène IV est un composant présent dans l'ensemble des membranes basales, la matrice extracellulaire sous-jacente aux cellules épithéliales et endothéliales qui entoure le muscle, la graisse et les cellules nerveuses. Il peut être utilisé comme un fin revêtement à la surface des cultures tissulaires afin d'améliorer la prolifération et la fixation des cellules et d'étudier ses effets sur le comportement cellulaire.
UOM:
1 * 1 mg
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