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Fournisseur:  OMEGA BIO-TEK
Description:   E.Z.N.A.® Plasmid Midi Kit propose une méthode fiable et novatrice pour isoler jusqu'à 200 µg d'ADN plasmidique en moins de 60 minutes. Ce kit associe la technologie de membrane HiBind® à la cohérence durable de la lyse alcaline SDS de cellules bactériennes afin d'offrir de l'ADN plasmidique de haute qualité. Les colonnes HiBind® DNA midi exemptes de DNase/RNase fonctionnent en éliminant les processus fastidieux d'extractions de phénol/chloroforme et de précipités d'alcool. La purification plasmidique faisant appel au E.Z.N.A.® Plasmid Midi Kit suit une procédure simple de liaison, lavage et d'élution permettant le traitement de plusieurs échantillons. Bien que les rendements varient en fonction du nombre de copies d'ADN, la souche <i>E. coli</i>, et les conditions de croissance, 50 ml d'une culture sur une nuit dans un milieu LB produit généralement 200 µg de nombre élevé de copies d'ADN plasmidique. Lorsque vous travaillez avec un faible nombre de copies de plasmides, le volume de départ de la culture peut être augmenté jusqu'à 100 ml. Un ADN de haute qualité est prêt pour une utilisation immédiate dans les applications de biologie moléculaire, telles que le séquençage automatisé fluorescent, la restriction de la digestion enzymatique, le dépistage et la transfection.
Numéro de catalogue: (22003.)

Fournisseur:  Biotium
Description:   En PE, à usage unique, stérile. Mini grattoirs pour la récolte de cellules ou lysats cellulaires en microplaques à puits.
UOM:  1 * 200 ST
Numéro de catalogue: (PRSI5255)

Fournisseur:  ProSci Inc.
Description:   SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.
UOM:  1 * 1 EA
Fournisseur:  MP Biomedicals
Description:   Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).
Fournisseur:  ProSci Inc.
Description:   The H57-597 monoclonal antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The crosslinking induces activation and proliferation of T cells, as a plate-bound or a soluble H57-597, or the plate-bound antibody can induce apoptosis, based on assay conditions. The beta chain of the TCR can combine with the alpha chain of the receptor to produce the alpha-beta TCR, which is expressed by the NKT cells, by the NK1.1+ thymocytes and most of the T cells. The beta chain does not react with the gamma-delta TCR-bearing cells, expressed by a small number of T cells. This antibody can be used as a phenotypic marker for the TCR beta expressing cells or for the functional purpose of TCR-mediated cell activation or apoptosis.
UOM:  1 * 0,025 mg
New Product
Fournisseur:  MP Biomedicals
Description:   Synonym: N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid)
Cell Culture Reagent
Crystalline Free Acid Zwitterionic Buffer useful in the pH range 6.8-8.2. pKa=7.55 at 25°C
Fournisseur:  STEMCELL Technologies
Description:   Betacellulin is a member of the epidermal growth factor (EGF) family, and signals through EGF receptor and ERBB4. It activates ERK and AKT pathways, which induces neural stem cell proliferation and prevents spontaneous differentiation in culture. Betacellulin stimulates the expansion of neural stem cells, transit-amplifying cells, and neuroblasts derived from subventricular zone and dentate gyrus (Gómez-Gaviro <i>et al.</i>). It is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Betacellulin down-regulates E-cadherin expression in ovarian cancer cell lines via MEK/ERK1/2 and PI3K/AKT signaling pathways, thus increasing cell migration (Zhao <i>et al.</i>). It is a modulator of interferon (IFN) response and enhances anti-viral effects of IFN (Al-Yahya <i>et al.</i>). Betacellulin is expressed in pancreatic α cells, β cells, and duct cells. It induces the proliferation of pancreatic cancer cell lines, inhibits apoptosis, promotes the neogenesis of β cells, and converts non-β cells into insulin-producing cells (Kawaguchi <i>et al.</i>; Miyagawa al.; Saito <i>et al.</i>).

Fournisseur:  ProSci Inc.
Description:   The PK136 monoclonal antibody is specific for the mouse NK1.1, a receptor from the killer cell lectin-like receptor (KLR) family. Nk1.1 is an antigen encoded by the Klrb1c/NKR-P1C gene expressed by the natural killer cells of some selected strains of mice (C57BL, FVB/N, NZB) and encoded by the Klrb1b/NKR-P1B gene expressed on Swiss NIH and SJL mice. Pk136 binds to an epitope common to NKR-P1B and NKR-P1C.The Klrb1 is a family of type II transmembrane C-type lectin receptors. Klrb1c activates the NK-cell cytotoxicity, while Klrb1b inhibits it. PK136 is useful in defining the NK cells and the rare population of NK-T lymphocytes and specific cultured monocytes.
UOM:  1 * 0,1 mg
New Product

Fournisseur:  ProSci Inc.
Description:   The PK136 monoclonal antibody is specific for the mouse NK1.1, a receptor from the killer cell lectin-like receptor (KLR) family. Nk1.1 is an antigen encoded by the Klrb1c/NKR-P1C gene expressed by the natural killer cells of some selected strains of mice (C57BL, FVB/N, NZB) and encoded by the Klrb1b/NKR-P1B gene expressed on Swiss NIH and SJL mice. Pk136 binds to an epitope common to NKR-P1B and NKR-P1C.The Klrb1 is a family of type II transmembrane C-type lectin receptors. Klrb1c activates the NK-cell cytotoxicity, while Klrb1b inhibits it. PK136 is useful in defining the NK cells and the rare population of NK-T lymphocytes and specific cultured monocytes.
UOM:  1 * 0,025 mg
New Product

Fournisseur:  ProSci Inc.
Description:   The PK136 monoclonal antibody is specific for the mouse NK1.1, a receptor from the killer cell lectin-like receptor (KLR) family. Nk1.1 is an antigen encoded by the Klrb1c/NKR-P1C gene expressed by the natural killer cells of some selected strains of mice (C57BL, FVB/N, NZB) and encoded by the Klrb1b/NKR-P1B gene expressed on Swiss NIH and SJL mice. Pk136 binds to an epitope common to NKR-P1B and NKR-P1C.The Klrb1 is a family of type II transmembrane C-type lectin receptors. Klrb1c activates the NK-cell cytotoxicity, while Klrb1b inhibits it. PK136 is useful in defining the NK cells and the rare population of NK-T lymphocytes and specific cultured monocytes.
UOM:  1 * 0,1 mg
New Product
Numéro de catalogue: (PRSI5295)

Fournisseur:  ProSci Inc.
Description:   IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
UOM:  1 * 1 EA
Numéro de catalogue: (PRSI55-089)

Fournisseur:  ProSci Inc.
Description:   The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
UOM:  1 * 400 µl
New Product
Fournisseur:  MP Biomedicals
Description:   Glycine is a non-essential amino acid. Acts as an inhibitory neurotransmitter, particularly in brain stem and medulla. Shown to block NMDA-stimulated dopamine release in fetal mesencephalic cell cultures. Also reported to increase NMDA-receptor mediated synaptic potential in cortical neurons. It does not have a chiral center, so only amino acid with no asymmetric carbon. Therefore, it does not have a D- or L- isomer designation. This material is synthetic. It is the smallest of the 20 natural amino acids.
Numéro de catalogue: (PRSI50-257)

Fournisseur:  ProSci Inc.
Description:   Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
UOM:  1 * 100 µl
Numéro de catalogue: (786-411)

Fournisseur:  G-Biosciences
Description:   Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
UOM:  1 * 250 mL
Fournisseur:  STEMCELL Technologies
Description:   Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda <i>et al.</i>). GM-CSF was first purified from the culture of mouse lung tissue after lipopolysaccharide treatment. GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 cells (Francisco-Cruz <i>et al.</i>). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton <i>et al.</i>). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz <i>et al.</i>).
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