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Enzymen
Itemnummer:
(PRSI92-658)
Leverancier:
ProSci Inc.
Omschrijving:
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.
UOM:
1 * 50 µG
Itemnummer:
(PRSI92-657)
Leverancier:
ProSci Inc.
Omschrijving:
Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
UOM:
1 * 50 µG
Itemnummer:
(PRSI91-648)
Leverancier:
ProSci Inc.
Omschrijving:
Dipeptidase 1 (DPEP1) is a kidney membrane enzyme that belongs to the peptidase M19 family. DPEP1 is a homodimer and is inhibited by L-penicillamine. DPEP1 hydrolyses a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates. DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem. DPEP1 may play an important role in the regulation of leukotriene activity. DPEP1 expression in cancer is significantly higher than that in normal tissue. However, DPEP1 expression decreased with pathological differentiation, lymph-node and distant metastasis.
UOM:
1 * 50 µG
Itemnummer:
(PRSI91-622)
Leverancier:
ProSci Inc.
Omschrijving:
Carboxypeptidase M (CPM) belongs to the peptidase M14 family, and exists in cell membrane. The protein binds 1 zinc ion per subunit, and cleavage of C-terminal arginine or lysine residues from polypeptides. CPM specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localised degradation of extracellular proteins.
UOM:
1 * 50 µG
Itemnummer:
(PRSI92-369)
Leverancier:
ProSci Inc.
Omschrijving:
Thymidine kinase 1(TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser-13 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5'-phosphate with the help of ATP.
UOM:
1 * 50 µG
Itemnummer:
(PRSI91-411)
Leverancier:
ProSci Inc.
Omschrijving:
Carboxypeptidase B1 is an exopeptidase which specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis and pancreatic graft rejection.Human CPB1 consists of a signal peptide, a pro region, and a mature chain. The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.
UOM:
1 * 50 µG
Itemnummer:
(PRSI91-528)
Leverancier:
ProSci Inc.
Omschrijving:
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.
UOM:
1 * 50 µG
Itemnummer:
(PRSI92-134)
Leverancier:
ProSci Inc.
Omschrijving:
Caspase-10 (CASP10) is a 521 amino acid protein member of the Cysteine-Aspartic Acid Protease (Caspase) family. CASP10 contains two DED (Death Effector) domains and is detectable in most tissues. CASP10 cleavage by Granzyme B and autocatalytic activity generate the two active subunits: Caspase-10 subunit p23/17, Caspase-10 subunit p12. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the execution-phase of cell apoptosis, the initiation and execution. Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7). CASP10 cleaves and activates caspases 3 and 7, but itself is processed by caspase 8. Defects in CASP10 are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.
UOM:
1 * 50 µG
Itemnummer:
(PRSI90-362)
Leverancier:
ProSci Inc.
Omschrijving:
Nicotinate Phosphoribosyltransferase (NAPRTase) is involved in the biological processes of pyridine nucleotide biosynthesis and nicotinate nucleotide salvage and functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
UOM:
1 * 50 µG
Itemnummer:
(PRSI92-419)
Leverancier:
ProSci Inc.
Omschrijving:
Mouse carboxypeptidase A2(CPA2) is a secreted pancreatic procarboxy -peptidase which belongs to the peptidase M14 family. CPA2 consists of a signal peptide, a pro region and a mature chain. It can be activated after cleavage of the pro peptide. CPA2 cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan.
UOM:
1 * 50 µG
Itemnummer:
(A4289.0100)
Itemnummer:
(605204-1)
Leverancier:
Merck Millipore (Calbiochem‎)
Omschrijving:
Native thrombin from human plasma, bound to agarose beads.
UOM:
1 * 1 mL
Itemnummer:
(605206-100)
Leverancier:
Merck Millipore (Calbiochem‎)
Omschrijving:
Native thrombin from human plasma. Formulation useful for procedures in which the presence of citrate is undesirable.
UOM:
1 * 100 UE
Itemnummer:
(J76230.MC)
Leverancier:
Thermo Scientific
Omschrijving:
Used in the isolation of native, high molecular weight DNA and RNA.
UOM:
1 * 100 mg
Leverancier:
OMEGA BIO-TEK
Omschrijving:
For enzyme digestion during DNA and RNA preparation. Both OB Protease and Proteinase K offer broad substrate specificity with high activity for a wide range of salts, denaturant and detergent, pH, and temperature conditions. Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus <i>Tritirachiumalbum</i> and is particularly suitable for short digestion times. It possesses a high specific activity which remains stable over a wide range of temperatures and pH values with substantially increased activity at higher temperature.
Itemnummer:
(69182-3)
Leverancier:
Merck Millipore (Novagen)
Omschrijving:
Desoxyribonuclease I
UOM:
1 * 1.000 UE
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het
nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
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