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Extractiebuffers


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Extractiebuffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Leverancier:  G-Biosciences
Omschrijving:   Tissue PE LBâ„¢ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LBâ„¢ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LBâ„¢. Tissue PE LBâ„¢ has been tested for use with a wide variety of fresh and frozen animal tissues.

Leverancier:  G-Biosciences
Omschrijving:   Mammalian Cell PE LBâ„¢ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LBâ„¢ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LBâ„¢. Mammalian Cell PE LBâ„¢ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
UOM:  1 * 500 mL
Leverancier:  G-Biosciences
Omschrijving:   Bacterial PE LBâ„¢ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LBâ„¢ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LBâ„¢ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
Leverancier:  Thermo Fisher Scientific
Omschrijving:   Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.
Leverancier:  AAT BIOQUEST INC
Omschrijving:   Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.
New Product
Leverancier:  G-Biosciences
Omschrijving:   Yeast PE LBâ„¢ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LBâ„¢ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LBâ„¢ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Leverancier:  AAT BIOQUEST INC
Omschrijving:   All our ReadiUseâ„¢ reagents require minimal hands-on time.
UOM:  1 * 10 mL
New Product
Leverancier:  Thermo Fisher Scientific
Omschrijving:   This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
UOM:  1 * 500 mL
Leverancier:  OMEGA BIO-TEK
Omschrijving:   Elution buffer, Omega Bio-Tek
Leverancier:  Thermo Fisher Scientific
Omschrijving:   The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
Leverancier:  PanReac AppliChem
Omschrijving:   Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Leverancier:  Thermo Fisher Scientific
Omschrijving:   The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM:  1 * 250 mL

Leverancier:  Thermo Fisher Scientific
Omschrijving:   The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
UOM:  1 * 250 mL
Leverancier:  Thermo Fisher Scientific
Omschrijving:   The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.
Itemnummer: (786-PSB)

Leverancier:  G-Biosciences
Omschrijving:   FOCUSâ„¢ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
UOM:  1 * 50 mL
Leverancier:  OMEGA BIO-TEK
Omschrijving:   RBB Buffer, 4000 ml, Omega Bio-Tek
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het call nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
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