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Protein Extraction Kits
Leverancier:
G-Biosciences
Omschrijving:
Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
Itemnummer:
(MILF9051)
Leverancier:
Merck
Omschrijving:
The Minicon® Static concentrators to concentrate urine and cerebrospinal fluid to intensify proteins that indicate abnormal or pathological states prior to analysis by electrophoresis or immunoelectrophoresis. The B15 unit has 8 cells and can hold up to 5 ml of sample. The CS15 has 10 cells and is suited for volumes up to 2,5 ml. The absorbent pulls solvent and salts through the ultrafilter, concentrating the sample.
UOM:
1 * 50 Tests
Leverancier:
Thermo Fisher Scientific
Omschrijving:
B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).
Itemnummer:
(786-246)
Leverancier:
G-Biosciences
Omschrijving:
Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
UOM:
1 * 1 KIT
Itemnummer:
(786-018)
Leverancier:
G-Biosciences
Omschrijving:
FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
UOM:
1 * 1 KIT
Itemnummer:
(PIER88279)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM:
1 * 1 KIT
Itemnummer:
(ENZOENZ450160100)
Leverancier:
ENZO LIFE SCIENCES
Omschrijving:
Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.
UOM:
1 * 1 KIT
New Product
Leverancier:
G-Biosciences
Omschrijving:
The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Leverancier:
G-Biosciences
Omschrijving:
Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.
Leverancier:
G-Biosciences
Omschrijving:
FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.
Leverancier:
Thermo Fisher Scientific
Omschrijving:
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
Itemnummer:
(786-129)
Leverancier:
G-Biosciences
Omschrijving:
A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.
UOM:
1 * 1 KIT
Itemnummer:
(PIER84868)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.
UOM:
1 * 1 KIT
Itemnummer:
(PIER89842)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
UOM:
1 * 1 KIT
Itemnummer:
(PIER89881)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
UOM:
1 * 1 KIT
Itemnummer:
(786-241A)
Leverancier:
G-Biosciences
Omschrijving:
InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
UOM:
1 * 1 KIT
Bel voor prijs
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 nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11.
De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het
nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
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