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Solid+Phase+Extraction
Numéro de catalogue:
(BOSSBS-6941R-HRP)
Fournisseur:
Bioss
Description:
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
UOM:
1 * 100 µl
Numéro de catalogue:
(BOSSBS-6941R-FITC)
Fournisseur:
Bioss
Description:
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
UOM:
1 * 100 µl
Fournisseur:
MACHEREY-NAGEL
Description:
CHROMABOND® Na₂SO₄/Florisil® is a combination phase for SPE of hydrocarbons from water.
Fournisseur:
WATERS
Description:
These columns complement the ACQUITY BEH C18 and C8 phases and provide a necessary and powerful tool for HPLC method development. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds compared to straight chain alkyl columns.
Fournisseur:
JOUR RESEARCH (VICI)
Description:
The in-line PEEK guard cartridges extend the life of analytical columns by trapping contaminants from the sample and mobile phase, thereby preventing loss of retention. Only PEEK and titanium contacts the mobile phase and sample. This system is ideal for use with proteins or other biopolymers that may be contaminated or absorbed by steel surfaces.
Numéro de catalogue:
(92446-5ML)
Fournisseur:
Merck
Description:
Sodium methoxide may be used as a derivatisation reagent to enhance the sensitivity of detection for the subsequent determination of organonitrogen herbicides and fungicide analytes in food samples, and bound fatty acids from acylglycerols, alkylacylglycerols, galactosyldiacylglycerols, glycerophospholipids, and sterol esters by gas chromatography (GC) based techniques.
UOM:
1 * 5 mL
New Product
Fournisseur:
Apollo Scientific
Description:
A purified agar with a high gel strength and clarity for tissue culture and microbiological work. Minimal conc. of 5.0g/L to obtain solid gel.
Fournisseur:
SIGMA-ALDRICH MICROSCOPY
Description:
Alizarin has been used as a biological stain to dye cotton fabrics and is used as standard in UV-visible spectrophotometer.
Fournisseur:
VWR Collection
Description:
Plateau intermédiaire (cadre de tamis avec base solide), VWR®, Acier inoxydable, Ø: 300 mm, Hauteur: 60 mm
Fournisseur:
Thermo Scientific
Description:
High temperature adhesive for bonding ceramic to metal.
Fournisseur:
Merck
Description:
2-tert-Butylhydroquinone pour la synthèse, Sigma-Aldrich®
Numéro de catalogue:
(TCIAA5602-5ML)
Fournisseur:
TCI
Description:
N-Triméthylsilylacetamidate de triméthylsilyle 25% dans l'acétonitrile (TMS-BA) pour la chromatographie en phase gazeuse
UOM:
1 * 5 mL
Numéro de catalogue:
(ENZOBMLKC1400025)
Fournisseur:
ENZO LIFE SCIENCES
Description:
Potassium ionophore.
UOM:
1 * 25 mg
New Product
Fournisseur:
MACHEREY-NAGEL
Description:
Polar GC phase.
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