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cell+culture+flasks
Numéro de catalogue:
(710-1964)
Fournisseur:
BIOTOOL AG
Description:
Le système de gaz anaérobie BioTool est idéal pour la mise en culture de bactéries anaérobies, microaérophiles et capnophiles dans des récipients anaérobies communs. Vous pouvez créer des conditions atmosphériques dans le récipient en moins de 1 minute et choisir le niveau d'appauvrissement en oxygène. L'extraction des gaz peut être contrôlée pour réduire le risque de lyse des cellules. Le système PetriSphere se compose d'un contrôleur à microprocesseur, d'une pompe à vide à diaphragme et d'une connexion pour réservoir de gaz permettant de remplacer l'air par de l'azote ou du gaz carbonique. Il suffit d'appuyer sur le bouton de démarrage pour que le processus démarre et fonctionne de manière entièrement automatique.
UOM:
1 * 1 ST
Numéro de catalogue:
(PRSI4555)
Fournisseur:
ProSci Inc.
Description:
Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
UOM:
1 * 1 EA
Numéro de catalogue:
(734-0105)
Fournisseur:
Corning
Description:
Le collagène IV est un composant présent dans l'ensemble des membranes basales, la matrice extracellulaire sous-jacente aux cellules épithéliales et endothéliales qui entoure le muscle, la graisse et les cellules nerveuses. Il peut être utilisé comme un fin revêtement à la surface des cultures tissulaires afin d'améliorer la prolifération et la fixation des cellules et d'étudier ses effets sur le comportement cellulaire.
UOM:
1 * 250 µG
Fournisseur:
STEMCELL Technologies
Description:
Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Martin <i>et al.</i>; Kent <i>et al.</i>). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). SCF is also a primary growth and activation factor for mast cells and eosinophils. SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain. This product is animal component-free.
Fournisseur:
STEMCELL Technologies
Description:
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf and Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit <i>et al.</i>), the activation of metalloproteases that cleave VCAM-1 (Levesque <i>et al.</i>), and the release of norepinephrine from the sympathetic nervous system (Katayama <i>et al.</i>), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola <i>et al.</i>). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya <i>et al.</i>). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.
Numéro de catalogue:
(PRSI4557)
Fournisseur:
ProSci Inc.
Description:
Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
UOM:
1 * 1 EA
Numéro de catalogue:
(IDCPMS3XWAB)
Fournisseur:
Dino-Lite / IDCP B.V.
Description:
These stable vertical desktop stands are designed to be used with Dino-Lite digital microscopes. Available as a standard and an ESD-safe model.
UOM:
1 * 1 ST
Fournisseur:
Spectrum Chemical
Description:
HEPES, cGMP Manufactured is a zwitterionic biological buffer and is one of Good's buffers widely used in cell culture. Non-graded HEPES, cGMP Manufactured supplied by Spectrum is indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use.
Fournisseur:
MP Biomedicals
Description:
HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.
Numéro de catalogue:
(PRSI50-253)
Fournisseur:
ProSci Inc.
Description:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.
UOM:
1 * 100 µl
Fournisseur:
Sartorius
Description:
The Vivapure® AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for<i> in vitro</i> transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.
Fournisseur:
MP Biomedicals
Description:
Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells. Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles. Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.
Fournisseur:
MP Biomedicals
Description:
Trehalose Dihydrate is an osmolyte, a chemical chaperone. It contains many properties that allow it to stabilize partially unfolded protein molecules and inhibit protein aggregation. It include a low chemical reactivity, high glass transition temperature, existence of numerous polymorphs, nonreducing nature, and high affinity for water molecules. Additionally, Trehalose has been noted to bind to expanded polyglutamines and stabilize partially unfolded polyglutamine-containing proteins.
Numéro de catalogue:
(17-1128-01)
Fournisseur:
Cytiva
Description:
MAbTrap™ est un kit de chromatographie d'affinité pour la purification rapide et efficace des anticorps IgG monoclonaux et polyclonaux provenant des liquides ascites, du sérum et des surnageants de cultures cellulaires. Le kit MAbTrap™ contient le nécessaire pour effectuer une purification en une seule étape sans utiliser d'équipements compliqués. Il élimine également les processus fastidieux de préparation des tampons et le remplissage de colonnes.
UOM:
1 * 1 KIT
Fournisseur:
MP Biomedicals
Description:
Storage: Store at +4 °C, store under nitrogen
Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. The sulfhydryl (thiol) group (SH) of cysteine serves as a proton donor and is responsible for the biological activity of glutathione. Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds. Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.
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