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MP Biomedicals


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Fournisseur:  MP Biomedicals
Description:   3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620 to 650 nm. The TMB reaction may be stopped with 2 M H<sub>2</sub>SO<sub>4</sub> (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.
Fournisseur:  MP Biomedicals
Description:   Alkaline phosphatase is a phosphomonoesterase, used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.
Fournisseur:  MP Biomedicals
Description:   Calcein is an indicator for the determination of calcium by titration with EDTA. The excitation wavelength and emission wavelength are 495/515 nm.
Numéro de catalogue: (ICNA04802511)

Fournisseur:  MP Biomedicals
Description:   Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands.

Biochem/physiol Actions:
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
UOM:  1 * 10 mL
Fournisseur:  MP Biomedicals
Description:   Hydrocortisone, a primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency. Hydrocortisone, known more formally as cortisone (INN, USAN, BAN), is a steroid hormone, produced by the zona fasciculata of the adrenal gland. It is released in response to stress and a low level of blood glucocorticoids.
Fournisseur:  MP Biomedicals
Description:   Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein.
Fournisseur:  MP Biomedicals
Description:   Major electron acceptor molecule in biological oxidations. Acts as a coenzyme for hydride-transfer enzymes and a substrate for NAD(+)-consuming enzymes, which include ADP-ribose transferases, poly(ADP-ribose) polymerases, cADP-ribose synthases and sirtuins. Recently reported to be a P2Y11 agonist.
Fournisseur:  MP Biomedicals
Description:   Anionic detergent that may be used in place of SDS for electrophoresis in cold conditions.
Fournisseur:  MP Biomedicals
Description:   Diatomaceous earth, non-washed is used as a filtration aid. It is suitable for most routine filtrations. Highly Insoluble.
Fournisseur:  MP Biomedicals
Description:   Soluble in water (50 mg/ml); practically insoluble in methanol, ethanol, and ether.
Fournisseur:  MP Biomedicals
Description:   Acetylcholine chloride is a cholinergic neurotransmitter that can induce the opening of calcium channels, as well as act on nicotinic and muscarinic acetylcholine receptors.
Fournisseur:  MP Biomedicals
Description:   Catalase, fungal suspension from from <i>Aspergillus niger</i> is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits, each containing a heme group bound to its active site.
Fournisseur:  MP Biomedicals
Description:   Nisin is a polycyclic lantibiotic produced by <i>Lactococcus lactis</i>. It is an antibiotic with bactericidal action and is commonly used as a food preservative. It is also used as a selective agent in cell culture for the isolation of gram-negative bacteria, yeast, and moulds.
Fournisseur:  MP Biomedicals
Description:   Inhibitors: p-Chloromercuribenzoate, iodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).
Fournisseur:  MP Biomedicals
Description:   N-dodecyl-β-D-maltoside is used as a non-ionic detergent for stabilization and activation of enzymes and for membrane research.
Numéro de catalogue: (ICNA0856030)

Fournisseur:  MP Biomedicals
Description:   Anti-beta-galactosidase Goat Polyclonal Antibody
UOM:  1 * 2 mL
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