STEMCELL Technologies
Fournisseur:
STEMCELL Technologies
Description:
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.
Fournisseur:
STEMCELL Technologies
Description:
Immunomagnetic negative selection kit.
Numéro de catalogue:
(STMC09860)
Fournisseur:
STEMCELL Technologies
Description:
Animal component-free medium for culture and expansion of human erythroid cells.
UOM:
1 * 500 mL
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Fournisseur:
STEMCELL Technologies
Description:
Rat monoclonal IgM antibody against mouse CD49b (integrin α2), Pacific Blue™-conjugated.
Fournisseur:
STEMCELL Technologies
Description:
Immunomagnetic selection of human CD4+CD127lowCD25+ regulatory T cells using particle release technology
Numéro de catalogue:
(STMC100-1270)
Fournisseur:
STEMCELL Technologies
Description:
Trigger a variety of immunological responses with <i>E. coli</i> Lipopolysaccharide O55:B5 (S-form), a lipopolysaccharide (LPS) derived from the O55:B5 serotype of the Gram-negative bacteria and nbsp <i>Escherichia coli</i>. Composed of a lipid A, a core oligosaccharide, and an O antigen, LPS are glycolipid constituents that reside on the outer membranes of gram-negative bacteria (Kitchens RL <i>et al.</i>). LPS protects bacteria against bile salts and lipophilic antibiotics by maintaining the outer integrity of the cell membrane (Bäckhed F <i>et al.</i>). <i>E. coli</i> lipopolysaccharide O55:B5 (S-form), in particular, is predominantly recognized by toll-like receptor 4 (TLR4), which leads to the activation of NF-κβ, a protein complex which plays a key role in regulating immune response (Kuzmich N <i>et al.</i>). Activation of NF-κβ can trigger increased production of pro-inflammatory cytokines IL-1 and TNF-α by macrophages (Matuschak GM <i>et al.</i>). This LPS can also interact with CD14 to activate phospholipase Cγ2 and kinases of the Src family, trigger influxes of extracellular Ca2+, as well as calcineurin-dependent translocation of the nuclear factor of activated T cells (NFAT) family of transcription factors (Li CC <i>et al.</i>). When added to ImmunoCult™-SF macrophage medium (Catalog #10961), stimulation with lipopolysaccharide from <i>E. coli</i> (O55:B5) and IFN-γ supports the polarization to M1 (classically activated) macrophages. Warning: This product is highly pyrogenic. Avoid all means by which the product may enter the bloodstream.
UOM:
1 * 1 mL
New Product
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Numéro de catalogue:
(STMC100-1335)
Fournisseur:
STEMCELL Technologies
Description:
Use visfatin to catalyse the production of nicotinamide mononucleotide from nicotinamide (Revollo <i>et al.</i>). Nicotinamide mononucleotide is a precursor to nicotinamide adenine dinucleotide (NAD) that is vital to energy metabolism, cell death, and other cellular processes (Ying). Visfatin also acts as an immunomodulator by activating leukocytes and inducing the production of pro-inflammatory and anti-inflammatory cytokines (Moschen <i>et al.</i>), and there is evidence that visfatin can regulate insulin receptor signaling and insulin secretion (Brown <i>et al.</i>). Belonging to the nicotinate phosphoribosyltransferase (NAPRTase) family, Visfatin is an adipokine that is produced in adipocytes, leukocytes, and hepatocytes (Chiu <i>et al.</i>, Garten <i>et al.</i>, Kralisch <i>et al.</i>). It is active as a homodimer with each identical monomer consisting of two structural domains made up of 19 β-strands and 13 α-helices (Kim <i>et al.</i>). For consistency and reproducibility across your applications, visfatin from STEMCELL comes lyophilised with ≥90% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.
UOM:
1 * 1 EA
New Product
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Numéro de catalogue:
(STMC100-1389)
Fournisseur:
STEMCELL Technologies
Description:
Stimulating T cells with EBV (LMP2A) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP2A) peptide pool is a lyophilised mixture of 27 peptides from latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV; strain GD1), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 118 on LMP2A. LMP2A contributes to the survival of EBV-transformed B cells (Mancao and Hammerschmidt) and mediates partial escape of infected B cells from recognition by EBV-specific CD8+ T cells (Rancan et al). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
UOM:
1 * 1 PE
New Product
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Numéro de catalogue:
(STMC100-1390)
Fournisseur:
STEMCELL Technologies
Description:
Stimulating T cells with EBV (GP350/340) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (GP350/340) Peptide pool is a lyophilised mixture of 224 peptides from envelope glycoprotein GP350 (GP350/340) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 907 on GP350/340. GP350 meditates EBV attachment to B lymphocytes via binding to the cell surface receptor CR2 (also known as C3d receptor or CD21) (Nemerow <i>et al.</i>; Tanner <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
UOM:
1 * 1 PE
New Product
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Numéro de catalogue:
(STMC100-1386)
Fournisseur:
STEMCELL Technologies
Description:
Stimulating T cells with EBV (EBNA-3A) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (EBNA-3A) Peptide pool is a lyophilised mixture of 234 peptides from Epstein-Barr nuclear antigen 3 (EBNA-3A) of Epstein-Barr virus (EBV; strain B95-8) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 944 on EBNA-3A. EBNA-3A contributes to B cell growth transformation (Hertle <i>et al.</i>; Tomkinson <i>et al.</i>) and has been found to interact with host cell proteins, where it activates (Young <i>et al.</i>) or represses (Cludts and Farrell; Hickabottom <i>et al.</i>) transcription of specific cellular genes. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.
UOM:
1 * 1 PE
New Product
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Fournisseur:
STEMCELL Technologies
Description:
Immunomagnetic positive selection of human cells.
Fournisseur:
STEMCELL Technologies
Description:
Immunomagnetic positive selection of non-human primate cells.
Numéro de catalogue:
(STMC100-1079)
Fournisseur:
STEMCELL Technologies
Description:
Extraction immunomagnétique d'acides nucléiques totaux (ADN et ARN).
UOM:
1 * 1 EA
New Product
Il s'agit d'un élément MarketSource. Des frais supplémentaires peuvent s'appliquer.
Fournisseur:
STEMCELL Technologies
Description:
For detection and measurement of human immunoglobulin E.
Fournisseur:
STEMCELL Technologies
Description:
For detection and measurement of mouse interleukin 5.
Fournisseur:
STEMCELL Technologies
Description:
For detection and measurement of human interleukin 10.
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