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What if you could reduce failure risk due to faulty plasmids and speed up your discovery process?

Webinar

Tuesday, 26th September, 2023
15:00 to 16:00 CEST (Berlin, Paris, Madrid)

Replay

Plasmid constructs serve as critical tools in biologic production and other biotechnology and molecular applications. However, plasmids can harbour unwanted genetic errors that cause failed experiments, unnecessary troubleshooting, weeks or months of lost time and increased costs. 
Plasmid verification by DNA sequencing is a critical quality control step prior to downstream applications, but typically only a partial region of the construct is analysed with Sanger sequencing. This does not detect important genetic errors in other regions of the plasmid. 
To de-risk the process, it is important to resolve the complete plasmid sequence as a QC step prior to use for the development of biologics, viral vectors or other applications. 
Oxford Nanopore Technologies has introduced a new ‘gold standard’ for plasmid verification, with a simple, rapid, end-to-end solution. Compared to other verification methods, the nanopore sequencing workflow is easier, faster and provides more comprehensive information to verify your complete constructs and ensure robust quality control. 

In this webinar, you will: 

  • Hear why the scientific community is quickly moving towards routine whole-plasmid sequencing to reduce failure risk and speed up the discovery process 
  • Learn how plasmid sequencing with nanopore technology provides fast and comprehensive insight for plasmid verification that is otherwise obtained through multiple different time-consuming approaches  
  • See the steps for the “end-to-end" plasmid workflow, including library preparation, sequencing and analysis of complete constructs 

For more information, please contact us at webinar@avantorsciences.com

 

Presented by: 

Jemma Jordan

Jemma Jordan is a Director within the Product Management team at Oxford Nanopore and is responsible for their range of library preparation kits. She has a PhD in Biochemistry and over a decade of laboratory experience developing and optimising assays and workflows.

 

Stephen Rudd

Stephen Rudd is an Associate Director at Oxford Nanopore, where he is responsible for bioinformatic products. He has two decades of experience in genome bioinformatics support and works with the Customer Workflows team towards demystifying bioinformatics.