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Biotium
Fournisseur:
Biotium
Description:
Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.
Fournisseur:
Biotium
Description:
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
Fournisseur:
Biotium
Description:
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
Numéro de catalogue:
(41020.)
Fournisseur:
Biotium
Description:
DNAzure® Blue Nucleic Acid Gel Stain is an ultrasensitive reagent for visible staining of dsDNA in agarose or polyacrylamide gels.
UOM:
1 * 10 mL
Fournisseur:
Biotium
Description:
The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.
Fournisseur:
Biotium
Description:
Cytotoxic agent that binds and inhibits dihydrofolate reductase. Useful for studying antimetabolite resistance, spontaneous gene amplification, and drug binding. Available conjugated to fluorescein and CF® Dyes.
Numéro de catalogue:
(30127.)
Fournisseur:
Biotium
Description:
The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.
UOM:
1 * 1 Un.
New Product
Numéro de catalogue:
(00023.)
Fournisseur:
Biotium
Description:
Fluorescein methotrexate is useful for studying anti-metabolite resistance and spontaneous gene amplification. The dye is actively taken up by cells and binds to dihydrofolate reductase (DHFR) quantitatively.
UOM:
1 * 1 mg
Numéro de catalogue:
(50011.)
Fournisseur:
Biotium
Description:
Fluo-3, pentapotassium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.
UOM:
1 * 1 mg
Numéro de catalogue:
(90041.)
Fournisseur:
Biotium
Description:
5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling.
UOM:
1 * 25 mg
Numéro de catalogue:
(90009.)
Fournisseur:
Biotium
Description:
Free acid form of 5-TAMRA (5-Carboxytetramethylrhodamine, single isomer).
UOM:
1 * 10 mg
Numéro de catalogue:
(90000.)
Fournisseur:
Biotium
Description:
For some applications, 5-(and-6)-carboxyrhodamine (5(6)-CR 110) is a superior alternative to the widely used green fluorescent dye 5-(and-6)-carboxyfluorescein. The dye is insensitive to pH between 4 and 9 and is much more photostable than fluorescein.
UOM:
1 * 10 mg
Numéro de catalogue:
(51017.)
Fournisseur:
Biotium
Description:
This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label. Carboxy-2′,7′-dichlorofluorescein is significantly more photo-stable than carboxyfluorescein.
UOM:
1 * 100 mg
Numéro de catalogue:
(90040.)
Fournisseur:
Biotium
Description:
5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
UOM:
1 * 25 mg
Numéro de catalogue:
(40020-1)
Fournisseur:
Biotium
Description:
5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.
UOM:
1 * 1 mg
Fournisseur:
Biotium
Description:
Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).
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