Reagentia voor eiwitextractie
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Surfact-Ampsâ„¢ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
Itemnummer:
(786-792)
Leverancier:
G-Biosciences
Omschrijving:
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM:
1 * 1 ST
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The carbonyl-reactive Thermo Scientificâ„¢ aminoxyTMTâ„¢ (Tandem Mass Tagâ„¢) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).
Itemnummer:
(PIER28340)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Surfact-Ampsâ„¢ Detergent sampler is a collection of various Surfact-Ampsâ„¢ detergent solutions and selected other useful detergents for protein research methods.
UOM:
1 * 1 KIT
Itemnummer:
(PIER20230)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierceâ„¢ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM:
1 * 2 mL
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierceâ„¢ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
Itemnummer:
(324713-50)
Leverancier:
Merck Millipore (Calbiochem‎)
Omschrijving:
Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM:
1 * 50 µG
Leverancier:
Merck Millipore (Novagen)
Omschrijving:
PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.
Itemnummer:
(324708-2)
Leverancier:
Merck Millipore (Calbiochem‎)
Omschrijving:
Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM:
1 * 2 µG
Leverancier:
Merck Millipore (Novagen)
Omschrijving:
The CytoBusterâ„¢ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
Leverancier:
Merck Millipore (Novagen)
Omschrijving:
BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.
Itemnummer:
(786-680)
Leverancier:
G-Biosciences
Omschrijving:
Protein-OUTâ„¢ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUTâ„¢ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUTâ„¢ removes inadvertent contamination with dust and keratin.
UOM:
1 * 50 mL
Leverancier:
VARIAN
Omschrijving:
Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.
Itemnummer:
(CEMATMS001-4X4L)
Leverancier:
CEM
Omschrijving:
Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.
UOM:
1 * 4 L
New Product
Itemnummer:
(WATE186001861)
Leverancier:
WATERS
Omschrijving:
RapiGestâ„¢ SF is an anionic surfactant for peptide digestion. Supplied as five 1 mg vials for 1 to 50 digestions. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes.
UOM:
1 * 5 ST
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
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