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Reagentia voor eiwitextractie


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Reagentia voor eiwitextractie

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Leverancier:  Thermo Fisher Scientific
Omschrijving:   Surfact-Ampsâ„¢ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
Itemnummer: (786-792)

Leverancier:  G-Biosciences
Omschrijving:   Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM:  1 * 1 ST
Leverancier:  Thermo Fisher Scientific
Omschrijving:   The carbonyl-reactive Thermo Scientificâ„¢ aminoxyTMTâ„¢ (Tandem Mass Tagâ„¢) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Leverancier:  Thermo Fisher Scientific
Omschrijving:   Surfact-Ampsâ„¢ Detergent sampler is a collection of various Surfact-Ampsâ„¢ detergent solutions and selected other useful detergents for protein research methods.
UOM:  1 * 1 KIT

Leverancier:  Thermo Fisher Scientific
Omschrijving:   Pierceâ„¢ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM:  1 * 2 mL
Leverancier:  Thermo Fisher Scientific
Omschrijving:   Pierceâ„¢ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Leverancier:  Merck Millipore (Calbiochem‎)
Omschrijving:   Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM:  1 * 50 µG
Leverancier:  Merck Millipore (Novagen)
Omschrijving:   PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.

Leverancier:  Merck Millipore (Calbiochem‎)
Omschrijving:   Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM:  1 * 2 µG
Leverancier:  Merck Millipore (Novagen)
Omschrijving:   The CytoBusterâ„¢ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
Leverancier:  Merck Millipore (Novagen)
Omschrijving:   BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozymeâ„¢ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Leverancier:  G-Biosciences
Omschrijving:   Protein-OUTâ„¢ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUTâ„¢ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUTâ„¢ removes inadvertent contamination with dust and keratin.
UOM:  1 * 50 mL
Leverancier:  VARIAN
Omschrijving:   Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

Leverancier:  CEM
Omschrijving:   Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.
UOM:  1 * 4 L
New Product
Itemnummer: (WATE186001861)

Leverancier:  WATERS
Omschrijving:   RapiGestâ„¢ SF is an anionic surfactant for peptide digestion. Supplied as five 1 mg vials for 1 to 50 digestions. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes.
UOM:  1 * 5 ST
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het call nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
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