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Biotium


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Fournisseur:  Biotium
Description:   Anti-IgG Rabbit Polyclonal Antibody (CF™ 488A)
Fournisseur:  Biotium
Description:   Anti-IgG2b Goat Polyclonal Antibody (CF™ 640R)
Fournisseur:  Biotium
Description:   Anti-IgG2a Goat Polyclonal Antibody (CF™ 350)
Fournisseur:  Biotium
Description:   Anti-IgG Chicken Polyclonal Antibody (CF™ 594)
Fournisseur:  Biotium
Description:   Anti-IgG2b Goat Polyclonal Antibody (CF™ 680)
Fournisseur:  Biotium
Description:   Anti-IgG Chicken Polyclonal Antibody (CF™ 488A)
Fournisseur:  Biotium
Description:   Anti-IgG Goat Polyclonal Antibody (Biotin)
Fournisseur:  Biotium
Description:   Anti-IgG Goat Polyclonal Antibody (CF™ 640R)

Fournisseur:  Biotium
Description:   5-FAM-PEO8 SE (full name: 5-Carboxyfluorescein-PEO8 propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group.
UOM:  1 * 1 mg
Fournisseur:  Biotium
Description:   5-ROX-PEO8 SE (full name: 5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group.
UOM:  1 * 1 mg
Fournisseur:  Biotium
Description:   SR101-PEO8 SE (Sulforhodamine 101-PEO8-propionic acid succinimydyl ester; Texas Red®-PEO8 SE) has an approximately 32 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.
UOM:  1 * 1 mg

Fournisseur:  Biotium
Description:   The principle of DPA/Tb3 for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb3 -DPA complex that is ~10,000 times more fluorescent than free Tb3 . In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl.
UOM:  1 * 1 SET
Numéro de catalogue: (92224.)

Fournisseur:  Biotium
Description:   This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
UOM:  1 * 1 KIT
Fournisseur:  Biotium
Description:   Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).
Fournisseur:  Biotium
Description:   This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF alpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Fournisseur:  Biotium
Description:   This antibody recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. ,The nuclear envelope (also known as the perinuclear envelope, nuclear membrane, nucleolemma or karyotheca) is the double membrane of the nucleus that encloses genetic material in eukaryotic cells. It separates the contents of the nucleus (DNA in particular) from the cytosol (cytoplasm). Numerous nuclear pores are present on the nuclear envelope to facilitate and regulate the exchange of materials (for example, proteins and RNA) between the nucleus and the cytoplasm. The space between the two membranes that make up the nuclear envelope is called the perinuclear space (also called the perinuclear cisterna), and is usually about 20 - 40 nm wide. Each of the two membranes is composed of a lipid bilayer. The outer membrane is continuous with the rough endoplasmic reticulum. The inner membrane is erected upon the nuclear lamina, a network of intermediate filaments made of lamin, that plays a role in mitosis and meiosis. The type of lamins present are A, B1, B2, and C. The nuclear envelope may also play a role in the disposition of chromatin inside the nucleus. The lamina acts as a site of attachment for chromosomes. It also acts like a shield for the nucleus. During prophase in mitosis, the chromatids begin condensing to form chromosomes, and the nuclear envelope begins to disintegrate. During metaphase, the nuclear envelope is completely disintegrated, and the chromosomes can be pulled apart as chromatids by the spindle fibers.
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