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Western Blotting Reagents


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Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Leverancier:  Thermo Scientific
Omschrijving:   Saponin permeating solution, is used as a cell permeabilising agent.
UOM:  1 * 250 mL

Leverancier:  Merck Millipore (Oncogene)
Omschrijving:   This Western-Re-Probeâ„¢ reagent is validated for use in immunoblotting.
UOM:  1 * 100 mL
Leverancier:  Thermo Scientific
Omschrijving:   For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.
Itemnummer: (TB110)

Leverancier:  G-Biosciences
Omschrijving:   Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, <i>E. coli</i>, Rhizobium and green algae.
UOM:  1 * 1 ST
Leverancier:  Thermo Fisher Scientific
Omschrijving:   The Fast Western Blot Kits, SuperSignalâ„¢ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignalâ„¢ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

Leverancier:  PanReac AppliChem
Omschrijving:   The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.
UOM:  1 * 1 KIT
Leverancier:  Rockland Immunochemicals
Omschrijving:   These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECLâ„¢ substrate is the equivalent of a 1:5000 dilution using the FemtoMaxâ„¢ substrate.

Leverancier:  Thermo Fisher Scientific
Omschrijving:   QuantaRedâ„¢ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.
UOM:  1 * 1 ST
Leverancier:  Cytiva
Omschrijving:   The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Leverancier:  Cytiva
Omschrijving:   ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM:  1 * 1 ST
Leverancier:  Cytiva
Omschrijving:   AlkPhos Directâ„¢ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Directâ„¢ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Leverancier:  Cytiva
Omschrijving:   Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Leverancier:  Cytiva
Omschrijving:   Chemiluminescence detection reagent for detection of high to medium abundant proteins.
Leverancier:  Cytiva
Omschrijving:   Cyâ„¢ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Leverancier:  Cytiva
Omschrijving:   ECFâ„¢ substrate for Western blotting
UOM:  1 * 1 ST
Leverancier:  Cytiva
Omschrijving:   ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
UOM:  1 * 1 ST
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De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het call nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
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