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Western Blotting Reagents
Leverancier:
Thermo Fisher Scientific
Omschrijving:
SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.
Itemnummer:
(PIER34035)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
UOM:
1 * 1 g
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
Itemnummer:
(786-423)
Leverancier:
G-Biosciences
Omschrijving:
High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
UOM:
1 * 1 L
Itemnummer:
(PIER34070)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
UOM:
1 * 100 mL
Itemnummer:
(PIER34000)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
UOM:
1 * 1 KIT
Itemnummer:
(PIER15162)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
UOM:
1 * 1 KIT
Itemnummer:
(PIER34042)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
UOM:
1 * 250 mL
Leverancier:
AAT BIOQUEST INC
Omschrijving:
Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.
Itemnummer:
(PIER37574)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.
UOM:
1 * 250 mL
Leverancier:
Thermo Fisher Scientific
Omschrijving:
LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.
Itemnummer:
(PIER34012)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
UOM:
1 * 250 mL
Itemnummer:
(PIER31490)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
UOM:
1 * 10 mg
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 nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11.
De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het
nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
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