A UHPLC/HPLC Method Development Strategy with Complementary Stationary Phases to Maximise Selectivity and ResolutionWebinarREPLAYIn this seminar, we review the importance of chromatographic selectivity in RPLC from a theoretical and practical perspective and how this relates to analyte resolution for method development. With an understanding of selectivity, and using a variety of chromatographic data, we discuss phase design principles and how it is possible to introduce functionality to enhance selectivity through mechanisms such as hydrophobicity, π-π, dipole-dipole and shape selectivity interactions. An overview of the method development workflow is discussed. Based upon the key parameters to maximise selectivity, a systematic and optimised method development screening platform is described and an example related substances method development activity is illustrated using the complementary stationary phases with MeOH and MeCN solvents. Presented by:
Alan McKeown As an experienced chromatographer in his own right, Alan has been invited and delivered chromatography technical seminars in Europe, Asia, North America and South America at conferences, customer sites and organised events. |
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