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Oligonucleotide analysis: Practical implementation approaches for HPLC and LC-MS analyses
Oligonucleotides represent a diverse class of therapeutics that provide great promise to treat disease, but can pose a number of analytical challenges to scientists. In this webinar, we will present:
Ion exchange and Reversed-Phase-Ion-Pair Approaches to the separation of Nucleic Acids
Separation examples of from small modified RNA, phosphothioated oligonucleotides, plasmid uncoiled and supercoiled DNA separations
Small scale purification options
LC/MS analysis of oligonucleotides
Thermo Scientific has over 20 years of innovations and publications around the separation of oligonucleotides, please join our webinar to learn how the DNAPac line of Ion Exchange and Reversed Phase columns can optimize and enhance your nucleic acid separations.
Presented by:
Dr. Ken Cook
Dr. Ken Cook is a bio-separations expert who began his career in as a biochemistry lecturer focused on protein chemistry and metabolism.
In well over 30 years of industry experience, Ken has covered a variety of analytical equipment, initially using HPLC, ion chromatography, SFC and capillary electrophoresis to develop new methods of analysis, including publications in a number of diverse fields. Currently, his role involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide-based bio-therapeutics as well as peptide mapping by LC/HRMS and charged variant analysis including novel coupling to MS. Recently, glycan analysis, aggregate analysis, on-line process monitoring and gene therapy characterisation is a growing interest.
