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Reagentia voor nucleïnezuren
Leverancier:
Thermo Fisher Scientific
Omschrijving:
The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.
Itemnummer:
(J60028.AK)
Leverancier:
Thermo Scientific
Omschrijving:
For transformation of competent cells.
UOM:
1 * 250 mL
Leverancier:
Thermo Fisher Scientific
Omschrijving:
GeneJET™ Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. GeneJET™ Plasmid Maxiprep Kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.
Leverancier:
G-Biosciences
Omschrijving:
GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.
Leverancier:
Sartorius
Omschrijving:
LentiSELECT kits offer LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 to 1000 ml cell culture, leading to purified infective particles. These lentivirus purification and concentration kits offer purified recombinant lentivirus particles for <i>in-vitro</i> transfection a fast, safe and easy to use solution. These kits replace time-consuming ultracentrifugation protocols, which typically take about one day for large sample volumes, thus reducing the purification time to only a few hours.
Leverancier:
G-Biosciences
Omschrijving:
GET™ DNA Template is a spin column format kit suitable for the preparation of DNA templates from blood, cells, fungal and animal tissue samples. The isolated genomic DNA is suitable for PCR and other downstream applications.
Leverancier:
MP Biomedicals
Omschrijving:
The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.
Itemnummer:
(K0761)
Leverancier:
Thermo Fisher Scientific
Omschrijving:
GeneJET™ Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.
UOM:
1 * 1 KIT
Leverancier:
G-Biosciences
Omschrijving:
Plasmid Screening ToothPick™ allows the rapid screening of bacteria for transformed plasmids. Simply pick a colony, add to Plasmid Screening ToothPick™ reagents and then analyse using restriction enzymes.
Itemnummer:
(786-398)
Leverancier:
G-Biosciences
Omschrijving:
OmniPrep™ for Gram Positive Bacteria Kit isolates high quality genomic DNA from gram-positive bacteria. If used according to the protocols, this kit purifies DNA from 100 ml gram-positive bacteria culture.
UOM:
1 * 1 KIT
Itemnummer:
(786-136)
Leverancier:
G-Biosciences
Omschrijving:
The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.
UOM:
1 * 1 KIT
Leverancier:
ZYGEM
Omschrijving:
prepGEM Universal is a manual extraction system used to extract DNA from saliva, buccal, dispersed cells, blood, EDTA-blood, insects, and most animal tissues.
Itemnummer:
(ZYGEPBA0500)
Leverancier:
ZYGEM
Omschrijving:
prepGEM® DNA extraction kits have been developed to provide a rapid, reliable, and economical solution to DNA extraction. The kits use a single tube protocol with temperature control to prepare DNA for analysis, and are optimised for downstream PCR-based applications, including qPCR, STR-PCR, DNA sequencing, AFLP analysis, and many SNiP detection applications.
UOM:
1 * 500 µl
Itemnummer:
(69831-3)
Leverancier:
Merck Millipore (Novagen)
Omschrijving:
The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.
UOM:
1 * 1 SET
Itemnummer:
(40078.)
Leverancier:
Biotium
Omschrijving:
Digoxigenin-dUTP, alkali stable
UOM:
1 * 25 Nmo
Leverancier:
G-Biosciences
Omschrijving:
<i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.
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 nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11.
De voorraad voor dit item is beperkt, maar kan beschikbaar zijn in een kortbijgelegen magazijn. Gelieve te controleren of u bent aangemeld op de site zodat de beschikbare voorraad weergegeven kan worden. Als het
nog steeds zichtbaar is en u hulp nodig heeft, gelieve VWR te contacteren via 016/38 50 11/
Dit product is gemarkeerd als beperkt en kan enkel gekocht worden door goedgekeurde verzendingsaccounts. Als u hulp nodig heeft gelieve dan het VWR-Regelgevend Departement te contacteren via regulatory_affairs@vwr.com.
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