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G Bioscienes
Numéro de catalogue:
(786-651)
Fournisseur:
G-Biosciences
Description:
β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.
UOM:
1 * 500 Essai
Numéro de catalogue:
(786-627)
Fournisseur:
G-Biosciences
Description:
For improved dialysis time and rates.
UOM:
1 * 50 mL
Numéro de catalogue:
(786-126)
Fournisseur:
G-Biosciences
Description:
The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
UOM:
1 * 1 KIT
Fournisseur:
G-Biosciences
Description:
Ultra pure DEPC-Treated water is suitable for use with sensitive RNA research. It is prepared by incubating with 0.1% DEPC (diethylpyrocarbonate), an RNase inhibitor, and then autoclaved to remove the DEPC, to prevent downstream interference, and sterilized.
Fournisseur:
G-Biosciences
Description:
Zwitterionic detergent, Aminosulfobetaine with C14 alkyl tail is useful for solubilizing proteins for 2D analysis. Optimal solubility achieved in urea-thiourea mixtures and not in urea alone.
Fournisseur:
G-Biosciences
Description:
GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
Numéro de catalogue:
(DG053)
Fournisseur:
G-Biosciences
Description:
CHAPSO
UOM:
1 * 5 g
Numéro de catalogue:
(786-791)
Fournisseur:
G-Biosciences
Description:
A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM:
1 * 1 ST
Numéro de catalogue:
(786-790)
Fournisseur:
G-Biosciences
Description:
A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM:
1 * 1 ST
Numéro de catalogue:
(BE-502)
Fournisseur:
G-Biosciences
Description:
Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
UOM:
1 * 1 KIT
Numéro de catalogue:
(786-488)
Fournisseur:
G-Biosciences
Description:
Potassium dihydrogénophosphate 1 mol/l en solution aqueuse
UOM:
1 * 1 L
Numéro de catalogue:
(786-048)
Fournisseur:
G-Biosciences
Description:
Chymostatin inhibits serine proteases having a chymotrypsin-like specificity, including α, β γ, and δ chymotrypsin, and most cysteine proteases including cathepsins B, H, L. Soluble in DMSO.
UOM:
1 * 5 mg
Numéro de catalogue:
(786-022)
Fournisseur:
G-Biosciences
Description:
FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM:
1 * 1 KIT
Numéro de catalogue:
(786-051)
Fournisseur:
G-Biosciences
Description:
Leupeptin, hemisulphate inhibits serine, plasmin, porcine kallikrein and cysteine proteases, including papain and cathepsin B. Does not inhibit chymotrypsin and thrombin
UOM:
1 * 25 mg
Numéro de catalogue:
(786-680)
Fournisseur:
G-Biosciences
Description:
Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
UOM:
1 * 50 mL
Numéro de catalogue:
(786-678)
Fournisseur:
G-Biosciences
Description:
Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.
UOM:
1 * 1 KIT
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